Enzymes of nucleic acid metabolism from mung bean sprouts. 3. The identification of 5'-mononucleotides as hydrolysis products of ribonuclease M-2.

نویسندگان

  • T L Walters
  • H S Loring
چکیده

The 3’nucleotidase present in mung bean sprouts was found to hydrolyze the d’-monophosphates of adenosine, guanosine, uridine, and cytidine in the order 3’-AMP > 3’GMP > 3’-UMP > 3’-CMP, and also to hydrolyze the 3’-phosphate group of coenzyme A, but showed no significant activity for 2’or S-ribonucleotides or for several non-nucleotide phosphomonoesters. pH optima and the respective K,,, values for 3’-AMP, 3’-GMP, 3’-CMP, and 3’-UMP were determined. The 3’nucleotidase and ribonuclease Mz were inactivated reversibly at pH 5 and by dialysis and irreversibly by ethylenediaminetetraacetate. Inactivation of both enzymes at pH 5 could be largely prevented by the presence of Zn++, while several other metal ions were ineffective. The optimal concentrations of Zn++ for preventing inactivation were considerably different for the 3’-nucleotidase and RNase Mz. The two enzyme activities were shnllarly inactivated by heat at pH 5 and 7.5. The pH optimum of RNase Mz was pH 5 with no Zn++ present, but dropped to pH 4.5 in the presence of 5 x 10d5 M Zn++, which also greatly increased its activity in the pH range 3.5 to 4.5. Several lines of evidence suggesting that the 3’-nucleotidase and RNase Mz activities may be due to the same protein are presented.

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عنوان ژورنال:
  • The Journal of biological chemistry

دوره 241 12  شماره 

صفحات  -

تاریخ انتشار 1966